6B5P

Structure of PfCSP peptide 20 with human antibody CIS42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-12
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	41.832, 70.680, 166.733
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.537 - 2.297
R-factor	0.2383
Rwork	0.237
R-free	0.26820
RMSD bond length	0.003
RMSD bond angle	0.642
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	32.537
High resolution limit [Å]	2.300
Number of reflections	18859
<I/σ(I)>	19.8
Completeness [%]	82.6
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% PEG3350, 9% Isopropanol, 0.12M Ammonium Citrate pH 8.5