6B5N
Structure of PfCSP peptide 25 with human protective antibody CIS43
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-28 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.068, 60.419, 83.307 |
Unit cell angles | 90.00, 102.83, 90.00 |
Refinement procedure
Resolution | 40.610 - 1.980 |
R-factor | 0.176 |
Rwork | 0.175 |
R-free | 0.20900 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.768 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.614 |
High resolution limit [Å] | 1.980 |
Number of reflections | 31446 |
<I/σ(I)> | 13.9 |
Completeness [%] | 99.6 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Sodium Hepes pH 7.5, 1.4M Sodium Citrate Tribasic Dihydrate |