6B5D

Structural Basis for Katanin Self-Assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 65
Unit cell lengths	98.830, 98.830, 75.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.442 - 3.100
R-factor	0.2106
Rwork	0.208
R-free	0.25460
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.729
Data reduction software	iMOSFLM
Data scaling software	XDS
Phasing software	SHELXCD
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	85.589	56.442	3.270
High resolution limit [Å]	3.100	9.800	3.100
Rmerge		0.018	0.624
Rmeas	0.112	0.054	0.778
Rpim	0.049	0.025	0.341
Total number of observations	37571	1192	5592
Number of reflections	7653	257	1118
<I/σ(I)>	13	41.1	2.4
Completeness [%]	99.6	97.7	100
Redundancy	4.9	4.6	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES pH 7.5 and 0.7 M Sodium potassium tartrate