6B57

tudor in complex with ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2017-01-17
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	47.070, 72.660, 130.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.350 - 1.930
R-factor	0.208
Rwork	0.206
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j39
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	Aimless (0.5.32)
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.350	65.350	1.980
High resolution limit [Å]	1.930	9.060	1.930
Rmerge	0.068	0.035	0.825
Rmeas	0.075	0.038	0.916
Rpim	0.029	0.016	0.387
Total number of observations	217894	2235	11705
Number of reflections	34211
<I/σ(I)>	15.2	34.7	1.9
Completeness [%]	99.5	98.4	97.4
Redundancy	6.4	5.7	5.2
CC(1/2)	0.998	0.997	0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% (w/v) PEG-3350, 0.2 M ammonium formate