6B4X
Schistosoma mansoni (Blood Fluke) Sulfotransferase, F39Y Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97890 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 139.726, 39.514, 53.784 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.863 - 1.460 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.19060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5byk |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.195 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.860 | 1.540 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rpim | 0.024 | 0.406 |
| Number of reflections | 52736 | 7581 |
| <I/σ(I)> | 17.6 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 1.0 M sodium citrate, 0.1 M sodium cacodylate, pH 6.5 |
