6B4H
Crystal structure of Chaetomium thermophilum Gle1 CTD-Nup42 GBM-IP6 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.160, 73.276, 117.665 |
Unit cell angles | 90.00, 94.20, 90.00 |
Refinement procedure
Resolution | 34.847 - 2.170 |
R-factor | 0.1944 |
Rwork | 0.193 |
R-free | 0.23020 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.533 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CRANK2 |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.250 |
High resolution limit [Å] | 2.170 | 2.170 |
Rpim | 0.043 | 0.498 |
Number of reflections | 52814 | 5249 |
<I/σ(I)> | 11.1 | 1.9 |
Completeness [%] | 98.4 | 98.5 |
Redundancy | 6.7 | 6.9 |
CC(1/2) | 0.999 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 10 mM zinc sulfate heptahydrate, 0.1 M MES pH 6.3, 18 % (w/v) PEG MME 550 |