6B4H

Crystal structure of Chaetomium thermophilum Gle1 CTD-Nup42 GBM-IP6 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	C 1 2 1
Unit cell lengths	119.160, 73.276, 117.665
Unit cell angles	90.00, 94.20, 90.00

Refinement procedure

Resolution	34.847 - 2.170
R-factor	0.1944
Rwork	0.193
R-free	0.23020
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.533
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.250
High resolution limit [Å]	2.170	2.170
Rpim	0.043	0.498
Number of reflections	52814	5249
<I/σ(I)>	11.1	1.9
Completeness [%]	98.4	98.5
Redundancy	6.7	6.9
CC(1/2)	0.999	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	10 mM zinc sulfate heptahydrate, 0.1 M MES pH 6.3, 18 % (w/v) PEG MME 550