6B4F
Crystal structure of human Gle1 CTD-Nup42 GBM complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.660, 69.710, 93.570 |
| Unit cell angles | 90.00, 90.66, 90.00 |
Refinement procedure
| Resolution | 46.782 - 2.811 |
| R-factor | 0.2469 |
| Rwork | 0.245 |
| R-free | 0.27350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.630 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.782 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rpim | 0.054 | 0.735 |
| Number of reflections | 25969 | 2551 |
| <I/σ(I)> | 10.1 | 1.1 |
| Completeness [%] | 99.2 | 97.8 |
| Redundancy | 3.8 | 3.5 |
| CC(1/2) | 0.992 | 0.536 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.2 M sodium potassium phosphate pH 7.6, 26 % (w/v) PEG 3350 |
