6B4C
Structure of Viperin from Trichoderma virens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2015-10-25 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 173.884, 209.566, 233.941 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.814 - 2.795 |
R-factor | 0.1904 |
Rwork | 0.190 |
R-free | 0.21070 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.457 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.795 | 2.795 |
Rmerge | 0.082 | |
Number of reflections | 209265 | |
<I/σ(I)> | 9 | |
Completeness [%] | 99.9 | |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 1.6-1.8 M ammonium sulfate, 100 mM sodium citrate, pH 5.5-5.7 |