6B42

Human Histidine Triad Nucleotide Binding Protein 1 (hHint1) 2'-deoxy-AMP complex at 1.13A resolution

Replaces:  5KM7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	39.738, 39.738, 141.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.283 - 1.130
R-factor	0.1543
Rwork	0.154
R-free	0.15990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1av5
RMSD bond length	0.006
RMSD bond angle	1.371
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.566	1.134
High resolution limit [Å]	1.130	1.130
Rmerge	0.074	0.584
Number of reflections	43018	350
<I/σ(I)>	16.9	2.1
Completeness [%]	98.7	79.5
Redundancy	10.6	4.3
CC(1/2)	1.000	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293	100 MM MES, 33% PEG 8000