6B3K
Crystal structure of mutant Spinach RNA aptamer in complex with Fab BL3-6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2015-11-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97920 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 147.216, 80.019, 93.405 |
Unit cell angles | 90.00, 111.47, 90.00 |
Refinement procedure
Resolution | 69.097 - 2.090 |
R-factor | 0.1938 |
Rwork | 0.193 |
R-free | 0.22580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kze |
RMSD bond length | 0.007 |
RMSD bond angle | 0.884 |
Data reduction software | HKL-2000 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 69.100 | 2.165 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.097 | 0.097 |
Number of reflections | 58291 | |
<I/σ(I)> | 12.3 | 1.1 |
Completeness [%] | 97.6 | 99.6 |
Redundancy | 6.9 | |
CC(1/2) | 0.998 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 296 | 8% Tacsimate (pH 7.0), 20% Polyethylene glycol 3,350, 0.1 M HEPES (pH 7.2) |