6B3K

Crystal structure of mutant Spinach RNA aptamer in complex with Fab BL3-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-11-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	C 1 2 1
Unit cell lengths	147.216, 80.019, 93.405
Unit cell angles	90.00, 111.47, 90.00

Refinement procedure

Resolution	69.097 - 2.090
R-factor	0.1938
Rwork	0.193
R-free	0.22580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kze
RMSD bond length	0.007
RMSD bond angle	0.884
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.100	2.165
High resolution limit [Å]	2.090	2.090
Rmerge	0.097	0.097
Number of reflections	58291
<I/σ(I)>	12.3	1.1
Completeness [%]	97.6	99.6
Redundancy	6.9
CC(1/2)	0.998	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	296	8% Tacsimate (pH 7.0), 20% Polyethylene glycol 3,350, 0.1 M HEPES (pH 7.2)