6B2X
Apo YiuA Crystal Form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-19 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.2828 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.515, 94.732, 171.564 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.465 - 2.199 |
R-factor | 0.199 |
Rwork | 0.196 |
R-free | 0.24570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4mo9 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.992 |
Data reduction software | HKL-2000 (v715) |
Data scaling software | HKL-2000 (v715) |
Phasing software | PHASER (2.7.16) |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.199 | 2.199 |
Rmerge | 0.057 | 0.248 |
Rpim | 0.036 | 0.162 |
Number of reflections | 34143 | 1605 |
<I/σ(I)> | 15.3 | |
Completeness [%] | 98.6 | 93.9 |
Redundancy | 3.2 | 3 |
CC(1/2) | 0.994 | 0.984 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 293 | 20% PEG 3350, 10 mM MES pH 5.5 |