6B2I
E45A mutant of the HIV-1 capsid protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033200 |
| Spacegroup name | P 6 |
| Unit cell lengths | 87.608, 87.608, 56.474 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.612 - 2.500 |
| R-factor | 0.2047 |
| Rwork | 0.202 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xfx |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.443 |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | PHENIX (1.10.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.940 | 37.940 | 2.600 |
| High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
| Rmerge | 0.144 | 0.043 | 0.807 |
| Rmeas | 0.162 | 0.049 | 0.916 |
| Rpim | 0.073 | 0.023 | 0.428 |
| Total number of observations | 41810 | 870 | 4139 |
| Number of reflections | 8647 | 197 | 942 |
| <I/σ(I)> | 9.1 | 25.7 | 2 |
| Completeness [%] | 99.3 | 97.5 | 95.9 |
| Redundancy | 4.8 | 4.4 | 4.4 |
| CC(1/2) | 0.992 | 0.998 | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG3350, NaI, Sodium cacodylate, Glycerol |
