6B2A
Crystal structure of fluoride channel Fluc Ec2 F80M Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99997 |
| Spacegroup name | P 41 |
| Unit cell lengths | 87.340, 87.340, 143.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.670 - 2.650 |
| R-factor | 0.2258 |
| Rwork | 0.225 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5kbn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.122 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.670 | 43.670 | 2.780 |
| High resolution limit [Å] | 2.650 | 8.790 | 2.650 |
| Rmerge | 0.094 | 0.041 | 1.425 |
| Rmeas | 0.099 | 0.044 | 1.517 |
| Rpim | 0.032 | 0.015 | 0.514 |
| Total number of observations | 8467 | 35820 | |
| Number of reflections | 31192 | 876 | 4121 |
| <I/σ(I)> | 14.4 | 44.4 | 1.5 |
| Completeness [%] | 100.0 | 98.6 | 100 |
| Redundancy | 9.9 | 9.7 | 8.7 |
| CC(1/2) | 0.997 | 0.995 | 0.667 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.2 | 295 | 30% PEG 600, 100 mM ADA, 10 mM HEPES, 100 mM NaF, 50 mM LiNO3 |
