6B26
Crystal structure of human STAC2 Tandem SH3 Domains (296-411)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.857, 47.993, 73.587 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.950 - 1.200 |
| R-factor | 0.1532 |
| Rwork | 0.152 |
| R-free | 0.16910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dl4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.321 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.950 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.024 | 0.201 |
| Number of reflections | 41292 | |
| <I/σ(I)> | 29.89 | 5.84 |
| Completeness [%] | 99.3 | 98.95 |
| Redundancy | 4.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.4 | 277.15 | 150mM KCl, 10mM HEPES, 2mM TCEP |
