6B26
Crystal structure of human STAC2 Tandem SH3 Domains (296-411)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.857, 47.993, 73.587 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.950 - 1.200 |
R-factor | 0.1532 |
Rwork | 0.152 |
R-free | 0.16910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dl4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.321 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.950 | 1.240 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.024 | 0.201 |
Number of reflections | 41292 | |
<I/σ(I)> | 29.89 | 5.84 |
Completeness [%] | 99.3 | 98.95 |
Redundancy | 4.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 7.4 | 277.15 | 150mM KCl, 10mM HEPES, 2mM TCEP |