6B21
Crystal structure of AmtB from E. coli bound to TopFluor cardiolipin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | H 3 2 |
Unit cell lengths | 138.780, 138.780, 159.140 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.219 - 2.450 |
R-factor | 0.1719 |
Rwork | 0.170 |
R-free | 0.21250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u7g |
RMSD bond length | 0.002 |
RMSD bond angle | 0.618 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.219 | 56.219 | 2.510 |
High resolution limit [Å] | 2.450 | 10.960 | 2.450 |
Rmerge | 0.065 | 0.019 | 0.490 |
Rmeas | 0.073 | 0.022 | 0.559 |
Number of reflections | 21583 | 260 | 1590 |
<I/σ(I)> | 14.72 | 48.94 | 2.85 |
Completeness [%] | 98.7 | 93.2 | 98.9 |
Redundancy | 4.547 | 4.331 | 4.168 |
CC(1/2) | 0.999 | 1.000 | 0.890 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 0.1M Tris, pH 8.0 0.3M Mg-nitrate 22% (w/v) PEG 8000 |