6B1Z

Crystal Structure of Glutamate-tRNA Synthetase from Elizabethkingia anophelis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	47.170, 99.780, 132.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.441 - 1.600
R-factor	0.1786
Rwork	0.178
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ja2
RMSD bond length	0.006
RMSD bond angle	0.763
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MR-Rosetta
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.441	44.441	1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.035	0.020	0.460
Rmeas	0.039	0.022	0.503
Total number of observations	503995
Number of reflections	83273	1067	6107
<I/σ(I)>	26.47	64.03	3.51
Completeness [%]	99.7	97.8	100
Redundancy	6.052	5.138	6.12
CC(1/2)	1.000	0.999	0.909

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	ElanA.01348.a.B1.PW38250 at 18.25 mg/ml was mixed 1:1 with JCSG+ (a5): 0.2 M magnesium formate, 20% (w/v) PEG-3350, cryoprotected with 20% ethylene glycol. Tray: 292004a5, puck: qrh6-5.