6B1Z
Crystal Structure of Glutamate-tRNA Synthetase from Elizabethkingia anophelis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.170, 99.780, 132.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.441 - 1.600 |
R-factor | 0.1786 |
Rwork | 0.178 |
R-free | 0.21060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ja2 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.763 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MR-Rosetta |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.441 | 44.441 | 1.640 |
High resolution limit [Å] | 1.600 | 7.160 | 1.600 |
Rmerge | 0.035 | 0.020 | 0.460 |
Rmeas | 0.039 | 0.022 | 0.503 |
Total number of observations | 503995 | ||
Number of reflections | 83273 | 1067 | 6107 |
<I/σ(I)> | 26.47 | 64.03 | 3.51 |
Completeness [%] | 99.7 | 97.8 | 100 |
Redundancy | 6.052 | 5.138 | 6.12 |
CC(1/2) | 1.000 | 0.999 | 0.909 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | ElanA.01348.a.B1.PW38250 at 18.25 mg/ml was mixed 1:1 with JCSG+ (a5): 0.2 M magnesium formate, 20% (w/v) PEG-3350, cryoprotected with 20% ethylene glycol. Tray: 292004a5, puck: qrh6-5. |