6B1I
Disrupted hydrogen bond network impairs ATPase activity in an Hsc70 cysteine mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.977 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.830, 79.196, 76.252 |
Unit cell angles | 90.00, 101.45, 90.00 |
Refinement procedure
Resolution | 40.727 - 2.300 |
R-factor | 0.1935 |
Rwork | 0.191 |
R-free | 0.24220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4h5r |
RMSD bond length | 0.002 |
RMSD bond angle | 0.471 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.500 | 2.300 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 40377 | |
<I/σ(I)> | 10.8 | |
Completeness [%] | 91.4 | |
Redundancy | 4.3 | |
CC(1/2) | 0.993 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | NaCl, Tris, PEG 3350, MgCl2, ATP |