6B15
Crystal structure of CBMbc (family CBM26) from Eubacterium rectale Amy13K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2015-04-18 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 131.730, 131.730, 151.870 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.020 - 2.100 |
R-factor | 0.2011 |
Rwork | 0.200 |
R-free | 0.22880 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.730 |
Data reduction software | iMOSFLM (7.1.2) |
Data scaling software | Aimless (0.5.8) |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.020 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.092 | 0.561 |
Rpim | 0.039 | 0.237 |
Number of reflections | 86483 | 8664 |
<I/σ(I)> | 7.86 | 2.03 |
Completeness [%] | 98.5 | 99.38 |
Redundancy | 6.5 | 6.4 |
CC(1/2) | 0.995 | 0.832 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 60% Tacsimate, 0.1 M Bis-Tris Propane, pH 7.0 |