6B0N

Crystal structure of the cleavage-independent prefusion HIV Env glycoprotein trimer of the clade A BG505 isolate (NFL construct) in complex with Fabs PGT122 and PGV19 at 3.39 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	293
Detector technology	PIXEL
Collection date	2015-10-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03321
Spacegroup name	P 63
Unit cell lengths	161.247, 161.247, 245.651
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.854 - 3.400
R-factor	0.3358
Rwork	0.335
R-free	0.34640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tvp
RMSD bond length	0.014
RMSD bond angle	1.726
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.510
High resolution limit [Å]	3.390	3.390
Rpim	0.145	0.559
Number of reflections	49063	4971
<I/σ(I)>	6.9	1.3
Completeness [%]	98.7	99.9
Redundancy	6.4	6.1
CC(1/2)	0.715	0.202

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1M HEPES (pH 7.0), 15% (w/v) PEG4000, and cryo-protected with 25% glycerol