6B0D

An E. coli DPS protein from ferritin superfamily

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9844
Spacegroup name	C 2 2 21
Unit cell lengths	117.625, 133.969, 139.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.247 - 1.500
R-factor	0.17796
Rwork	0.176
R-free	0.20767
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1f30
RMSD bond length	0.022
RMSD bond angle	2.018
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.247
High resolution limit [Å]	1.449
Number of reflections	190268
<I/σ(I)>	3.7
Completeness [%]	98.5
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	50 mM Sodium Acetate (pH: 5.9); 100 mM NaCl and 5% glycerol