6B03

The crystal structure of the ferredoxin protease FusC in complex with its substrate plant ferredoxin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-16
Detector	MAR CCD 130 mm
Wavelength(s)	0.987
Spacegroup name	P 2 21 21
Unit cell lengths	82.578, 127.260, 133.205
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.289 - 2.700
R-factor	0.1981
Rwork	0.196
R-free	0.24270
RMSD bond length	0.009
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.010	2.810
High resolution limit [Å]	2.700	2.700
Rmerge	0.149	1.363
Rpim	0.094	0.614
Number of reflections	39305	4382
<I/σ(I)>	7.2	1.2
Completeness [%]	100.0	100
Redundancy	6.6	6.9
CC(1/2)	0.995	0.538

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	20% PEG3350, 0.2 M NaKPO4, 0.1 M BisTrisPropane