6AZ4
RNA hairpin complex with guanosine dinucleotide ligand G(5')ppp(5')G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 99 |
Detector technology | CCD |
Collection date | 2017-09-07 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 79.388, 79.388, 73.652 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.980 |
R-factor | 0.18206 |
Rwork | 0.181 |
R-free | 0.23250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fnj |
RMSD bond length | 0.009 |
RMSD bond angle | 2.185 |
Data reduction software | HKL-2000 (v1.0) |
Data scaling software | HKL-2000 (v1.0) |
Phasing software | REFMAC (5.8.0158) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.090 |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.133 | 0.698 |
Number of reflections | 4712 | 476 |
<I/σ(I)> | 9.24 | 1.64 |
Completeness [%] | 91.6 | 95.2 |
Redundancy | 4.5 | 4.4 |
CC(1/2) | 0.996 | 0.978 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 0.05 M Magnesium chloride, 0.1 M Sodium cacodylate trihydrate pH 6.5, 1.4 M Sodium acetate trihydrate |