6AZ2
Crystal structure of Asf1-Fab 12E complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | .979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 99.666, 109.743, 122.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.055 - 2.477 |
R-factor | 0.1948 |
Rwork | 0.193 |
R-free | 0.22760 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.643 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.055 |
High resolution limit [Å] | 2.477 |
Number of reflections | 47748 |
<I/σ(I)> | 30.3 |
Completeness [%] | 98.8 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 20% PEG3350, 0.2 M ammonium acetate |