6AZ2

Crystal structure of Asf1-Fab 12E complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-03
Detector	ADSC QUANTUM 315
Wavelength(s)	.979
Spacegroup name	P 21 21 21
Unit cell lengths	99.666, 109.743, 122.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.055 - 2.477
R-factor	0.1948
Rwork	0.193
R-free	0.22760
RMSD bond length	0.003
RMSD bond angle	0.643
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2152: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.055
High resolution limit [Å]	2.477
Number of reflections	47748
<I/σ(I)>	30.3
Completeness [%]	98.8
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	298	20% PEG3350, 0.2 M ammonium acetate