6AY7
Cartilage homing cysteine-dense-peptides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-20 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.838, 47.125, 48.538 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.810 - 1.770 |
| R-factor | 0.14268 |
| Rwork | 0.140 |
| R-free | 0.19262 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6atm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.528 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.056 | 0.110 |
| Rpim | 0.025 | 0.106 |
| Number of reflections | 5643 | 34 |
| <I/σ(I)> | 32.4 | 8.4 |
| Completeness [%] | 80.9 | 9.9 |
| Redundancy | 5.4 | 1.6 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 298 | 0.2M Na chloride, 0.1M Phosphate-citrate pH 4.2, 20% PEG 8000 |
