6AXO
F96S Epi-isozizaene synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.920, 76.670, 108.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.668 - 2.100 |
R-factor | 0.1787 |
Rwork | 0.176 |
R-free | 0.22190 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kb9 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.753 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 62.670 |
High resolution limit [Å] | 2.100 |
Number of reflections | 23391 |
<I/σ(I)> | 6.1 |
Completeness [%] | 98.7 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 1.0 M ammonium citrate tribasic (pH 7.0), 0.1M bis-Tris propane (pH 7.0) |