6AXM
F95Y Epi-isozizaene synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-11 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.120, 46.830, 75.720 |
Unit cell angles | 90.00, 96.89, 90.00 |
Refinement procedure
Resolution | 51.740 - 1.800 |
R-factor | 0.172 |
Rwork | 0.170 |
R-free | 0.21410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kb9 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.830 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 51.740 |
High resolution limit [Å] | 1.800 |
Number of reflections | 32978 |
<I/σ(I)> | 7 |
Completeness [%] | 97.6 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.17 M sodium acetate trihydrate, 85 mM sodium cacodylate trihydrate (pH 6.5), 25.5% PEG 8000, 15% glycerol |