6AXL
Crystal structure of Fab317 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 82.663, 65.342, 99.043 |
Unit cell angles | 90.00, 106.40, 90.00 |
Refinement procedure
Resolution | 40.930 - 2.400 |
R-factor | 0.178 |
Rwork | 0.175 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology model |
RMSD bond length | 0.002 |
RMSD bond angle | 0.591 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.930 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rpim | 0.079 | 0.430 |
Number of reflections | 38939 | |
<I/σ(I)> | 11.2 | |
Completeness [%] | 97.8 | |
Redundancy | 6.1 | |
CC(1/2) | 0.920 | 0.732 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M Sodium Citrate pH4.0 1M Lithium Chloride 20% PEG6000 |