6AVX

Crystal structure of Arabidopsis thaliana SOBER1 F65L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	45.500, 52.080, 75.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.032 - 1.271
R-factor	0.1509
Rwork	0.150
R-free	0.16570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6avv
RMSD bond length	0.007
RMSD bond angle	0.949
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.032	1.320
High resolution limit [Å]	1.270	1.270
Rmerge	0.025	0.057
Number of reflections	47288	4531
<I/σ(I)>	18.77	9.09
Completeness [%]	98.0	95
Redundancy	2	1.9
CC(1/2)	0.998	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M MES pH 6.5, 16% (v/v) PEG 20,000