6AVV

Crystal structure of Arabidopsis thaliana SOBER1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	45.760, 52.290, 74.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.436 - 1.510
R-factor	0.17001982761
Rwork	0.169
R-free	0.19620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fj2
RMSD bond length	0.005
RMSD bond angle	0.858
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.440	1.564
High resolution limit [Å]	1.510	1.510
Rmerge	0.082	0.759
Rpim	0.023	0.232
Number of reflections	28632	2802
<I/σ(I)>	21.74	4.58
Completeness [%]	99.9	99.96
Redundancy	13.5	11.6
CC(1/2)	0.999	0.922

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	0.1 M MES pH 6.5, 16% (v/v) PEG 20,000