6AVH
GH3.15 acyl acid amido synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 180.911, 191.605, 319.364 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.528 - 3.011 |
| R-factor | 0.1875 |
| Rwork | 0.185 |
| R-free | 0.24940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4epl |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.405 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 159.682 | 3.090 |
| High resolution limit [Å] | 3.011 | 3.011 |
| Number of reflections | 54417 | |
| <I/σ(I)> | 14.9 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 25% PEG1500, 0.1 M MIB (sodium malonate, imidazole, boric acid), pH 5.0, 2 mM AMP |
