6AU8
1.8 angstrom crystal structure of the human Bag6-NLS & TRC35 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-14 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9200, 0.8154 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.660, 84.560, 102.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.090 - 1.800 |
R-factor | 0.1615 |
Rwork | 0.159 |
R-free | 0.20180 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.167 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.090 | 39.090 | 1.850 |
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.080 | 0.030 | 0.708 |
Rmeas | 0.085 | 0.032 | 0.747 |
Total number of observations | 323983 | ||
Number of reflections | 34348 | 454 | 2488 |
<I/σ(I)> | 19.15 | 55.22 | 3.46 |
Completeness [%] | 99.9 | 99.1 | 100 |
Redundancy | 9.432 | 8.427 | 9.795 |
CC(1/2) | 0.999 | 0.999 | 0.933 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 20% PEG-3350, 0.2 M DL-malic acid, pH 7.0 |