6ATX
Crystal structure of Physcomitrella patens KAI2-like C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 |
Unit cell lengths | 46.630, 46.630, 140.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.630 - 2.740 |
R-factor | 0.179600563238 |
Rwork | 0.178 |
R-free | 0.21901 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ih1 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.596 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.630 | 2.840 |
High resolution limit [Å] | 2.740 | 2.740 |
Rmerge | 0.220 | 0.834 |
Rpim | 0.062 | 0.296 |
Number of reflections | 7881 | |
<I/σ(I)> | 12.35 | |
Completeness [%] | 99.9 | |
Redundancy | 13.7 | |
CC(1/2) | 0.994 | 0.504 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 1.2 M ammonium sulfate, 0.2 M Bis-Tris, pH 6.5 |