6ATP
Crystal structure of apo-hGSTA1-1 exhibiting a new conformation of C-terminal helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-11-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.569, 91.442, 51.621 |
| Unit cell angles | 90.00, 92.38, 90.00 |
Refinement procedure
| Resolution | 36.572 - 1.700 |
| R-factor | 0.1679 |
| Rwork | 0.167 |
| R-free | 0.20110 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1guh |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.596 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.890 |
| Number of reflections | 49390 | 3829 |
| <I/σ(I)> | 28.72 | |
| Completeness [%] | 97.1 | 75.5 |
| Redundancy | 6.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 2000 MME, 20% (w/v), 0.05 M Tris HCl, pH 8.5 |
