6ATF
HLA-DRB1*1402 in complex with Vimentin59-71
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .95370 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.980, 77.770, 94.540 |
| Unit cell angles | 90.00, 109.52, 90.00 |
Refinement procedure
| Resolution | 33.482 - 1.900 |
| R-factor | 0.1715 |
| Rwork | 0.170 |
| R-free | 0.20230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mcy |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.913 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.482 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rpim | 0.056 | 0.230 |
| Number of reflections | 72027 | 10379 |
| <I/σ(I)> | 10.6 | 3.3 |
| Completeness [%] | 99.7 | 98.9 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 294 | 26% PEG 3350, 0.2M Potassium Nitrate, 0.1M Bis-Tris-Propane pH 7.3 |
