6AT8

1.1 Angstrom Resolution Structure of Human Cellular Retinol-Binding Protein IV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-08-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97919
Spacegroup name	C 1 2 1
Unit cell lengths	34.820, 57.334, 68.115
Unit cell angles	90.00, 103.09, 90.00

Refinement procedure

Resolution	29.190 - 1.105
R-factor	0.1267
Rwork	0.126
R-free	0.14920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lpj
RMSD bond length	0.007
RMSD bond angle	0.971
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.190	1.160
High resolution limit [Å]	1.105	1.105
Rmerge	0.053
Rmeas	0.035	0.090
Rpim	0.026	0.077
Number of reflections	49226	5474
<I/σ(I)>	31.8	8.6
Completeness [%]	94.4	72
Redundancy	5.5
CC(1/2)	0.994	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.2 M NaCl, 0.1 M Bis-Tris/HCl, 25% PEG3350 (w/v)