6AT1
STRUCTURAL CONSEQUENCES OF EFFECTOR BINDING TO THE T STATE OF ASPARTATE CARBAMOYLTRANSFERASE. CRYSTAL STRUCTURES OF THE UNLIGATED AND ATP-, AND CTP-COMPLEXED ENZYMES AT 2.6-ANGSTROMS RESOLUTION
Replaces: 4ATCExperimental procedure
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 122.000, 122.000, 142.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.18 * |
| Rwork | 0.160 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.500 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.600 * |
| Rmerge | 0.060 * |
| Total number of observations | 78896 * |
| Number of reflections | 26912 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 5.7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | maletic acid | 100 (mM) | |
| 2 | 1 | 1 | Tris-HCl | 10 (mM) | |
| 3 | 1 | 1 | 2-mercaptoethanol | 1 (mM) | |
| 4 | 1 | 1 | EDTA | 0.2 (mM) | |
| 5 | 1 | 1 | 0.3 (mM) | ||
| 6 | 1 | 2 | protein | 6 (mg/ml) |
