6ASR
REV1 UBM2 domain complex with ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 164.924, 164.924, 164.924 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.284 - 2.356 |
R-factor | 0.2025 |
Rwork | 0.201 |
R-free | 0.22910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4s1z |
RMSD bond length | 0.006 |
RMSD bond angle | 0.490 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 16003 | 781 |
<I/σ(I)> | 15.9 | |
Completeness [%] | 99.1 | 100 |
Redundancy | 6.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.1 M MES pH 6.0, 10 % PEG 8000 |