6ASM
E. coli phosphoenolpyruvate carboxykinase G209S K212C mutant bound to thiosulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.008 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.706, 94.080, 46.596 |
| Unit cell angles | 90.00, 96.40, 90.00 |
Refinement procedure
| Resolution | 47.040 - 1.550 |
| R-factor | 0.1702 |
| Rwork | 0.170 |
| R-free | 0.18810 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.089 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.040 |
| High resolution limit [Å] | 1.550 |
| Number of reflections | 145769 |
| <I/σ(I)> | 9.45 |
| Completeness [%] | 94.7 |
| Redundancy | 2.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.1 M Bis-Tris pH 5.5, 0.4 M sodium chloride |
