6ASK
Crystal Structure of apo Flavin monooxygenase CmoJ (earlier YtnJ)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 86.751, 86.751, 195.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.870 - 1.690 |
R-factor | 0.175 |
Rwork | 0.174 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yw1 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.750 |
Data scaling software | iMOSFLM (7.2.1) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.870 | 1.740 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.140 | 1.884 |
Number of reflections | 84371 | |
<I/σ(I)> | 12.1 | 1.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 21.2 | 23.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 50 mM HEPES pH7.5, 200 mM KCl, 29%-33% pentaerythritol propoxylate (5/4 PO/OH) |