6AS9

Filamentous Assembly of Green Fluorescent Protein Supported by a C-terminal fusion of 18-residues, viewed in space group P212121 form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	51.100, 54.520, 85.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.960 - 1.750
R-factor	0.1572
Rwork	0.156
R-free	0.18310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.100
Data reduction software	XDS (20160517)
Data scaling software	XSCALE (20160517)
Phasing software	PHASER (2.6.1)
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.960	45.960	1.800
High resolution limit [Å]	1.750	7.840	1.750
Rmerge	0.117	0.052	0.585
Rmeas	0.126	0.057	0.630
Total number of observations	175062
Number of reflections	24417	326	1743
<I/σ(I)>	14.07	28.9	3.76
Completeness [%]	99.4	99.7	98.5
Redundancy	7.17	6.132	7.009
CC(1/2)	0.996	0.996	0.857

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	277	0.1 M sodium acetate, pH 5.0 and 65% (v/v) 2-methyl-2,4-pentanediol