6ARZ
Structure of a phage anti-CRISPR protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2013-07-13 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.137, 63.959, 59.701 |
| Unit cell angles | 90.00, 100.94, 90.00 |
Refinement procedure
| Resolution | 48.177 - 2.500 |
| R-factor | 0.1815 |
| Rwork | 0.179 |
| R-free | 0.22080 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.177 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 24655 |
| <I/σ(I)> | 24.1 |
| Completeness [%] | 99.9 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 293 | 0.1M sodium cacodylate pH 6.1, 23% PEG 3350, 0.5M sodium bromide, and 7% glycerol |
