6AR5

Structure of a Thermostable Group II Intron Reverse Transcriptase with Template-Primer and Its Functional and Evolutionary Implications (Duplex Only)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9765
Spacegroup name	P 31 2 1
Unit cell lengths	46.428, 46.428, 82.195
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.118 - 2.413
R-factor	0.187
Rwork	0.185
R-free	0.21210
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.948
Data reduction software	XDS (May 1, 2016)
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.118	41.100	2.500
High resolution limit [Å]	2.410	9.020	2.410
Rmerge	0.053	0.021	0.531
Rmeas	0.055	0.022	0.544
Rpim	0.012	0.006	0.117
Total number of observations	88698
Number of reflections	4275	106	436
<I/σ(I)>	48
Completeness [%]	100.0	98.8	100
Redundancy	20.7	16	21.3
CC(1/2)	1.000	1.000	0.983

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium malonate, ammonium citrate tribasic