6AR3

Structure of a Thermostable Group II Intron Reverse Transcriptase with Template-Primer and Its Functional and Evolutionary Implications (RT/Duplex (Se-Met))

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9765
Spacegroup name	C 1 2 1
Unit cell lengths	179.527, 108.966, 72.517
Unit cell angles	90.00, 113.78, 90.00

Refinement procedure

Resolution	48.930 - 3.410
R-factor	0.2783
Rwork	0.273
R-free	0.32370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kyl 5irf
RMSD bond length	0.008
RMSD bond angle	1.258
Data reduction software	XDS (May 1, 2016)
Data scaling software	Aimless (0.5.31)
Phasing software	PHASER (2.7.16)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.930	48.930	3.690
High resolution limit [Å]	3.410	9.030	3.410
Rmerge	0.224	0.039	0.850
Rmeas	0.240	0.042	0.912
Rpim	0.087	0.016	0.330
Total number of observations	132418
Number of reflections	17494	977	3571
<I/σ(I)>	9.2
Completeness [%]	99.9	99.5	99.7
Redundancy	7.6	7.1	7.6
CC(1/2)	0.995	0.999	0.884

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris-HCl, sodium citrate tribasic dihydrate