6APO
Anti-Marburgvirus Nucleoprotein Single Domain Antibody A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97917 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.597, 49.362, 58.814 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.810 - 1.168 |
R-factor | 0.149 |
Rwork | 0.148 |
R-free | 0.17280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yc7 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.967 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.600 | 1.230 |
High resolution limit [Å] | 1.168 | 1.170 |
Rpim | 0.028 | 0.198 |
Number of reflections | 40045 | 4261 |
<I/σ(I)> | 17.6 | 3.4 |
Completeness [%] | 95.7 | 71.3 |
Redundancy | 6.6 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 25% polyethylene glycol 1000, 0.1M Tris-HCl pH 8.5 |