6APJ
Crystal Structure of human ST6GALNAC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | P 1 |
| Unit cell lengths | 71.346, 71.803, 134.246 |
| Unit cell angles | 98.78, 101.92, 103.48 |
Refinement procedure
| Resolution | 44.280 - 3.100 |
| R-factor | 0.2062 |
| Rwork | 0.201 |
| R-free | 0.25230 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.507 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.400 | 3.240 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.059 | 0.588 |
| Rpim | 0.059 | 0.416 |
| Number of reflections | 52564 | 4117 |
| <I/σ(I)> | 11.4 | 2.3 |
| Completeness [%] | 93.7 | 93.3 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.2 | 277 | 0.1M Sodium Citrate (pH 4.2), 0.1M Ammonium Sulfate, and 24% (w/v) PEG 20000 |
