6AOU

Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin in complex with 3'-SLNLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	H 3 2
Unit cell lengths	100.614, 100.614, 383.106
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.750
R-factor	0.16925
Rwork	0.168
R-free	0.19028
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o5n
RMSD bond length	0.012
RMSD bond angle	1.540
Data scaling software	HKL-2000 (712)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.810
High resolution limit [Å]	1.750	1.750
Rpim	0.020	0.300
Number of reflections	75864	7484
<I/σ(I)>	43	2.2
Completeness [%]	99.9	99.9
Redundancy	11.1	9.8
CC(1/2)	1.000	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.1 M bicine pH 9 and 41% 2-Methyl-2,4-pentanediol