6AOR
Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 100.556, 100.556, 384.183 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.17024 |
| Rwork | 0.169 |
| R-free | 0.19624 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o5n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.518 |
| Data scaling software | HKL-2000 (712) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rpim | 0.020 | 0.210 |
| Number of reflections | 81614 | 8029 |
| <I/σ(I)> | 51.1 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19 | 18.7 |
| CC(1/2) | 1.000 | 0.930 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 0.1 M bicine pH 9 and 41% 2-Methyl-2,4-pentanediol |
