6AOQ
Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | H 3 2 |
Unit cell lengths | 100.614, 100.614, 382.620 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.350 |
R-factor | 0.21791 |
Rwork | 0.215 |
R-free | 0.26157 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o5n |
RMSD bond length | 0.010 |
RMSD bond angle | 1.462 |
Data scaling software | HKL-2000 (712) |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Rpim | 0.030 | 0.220 |
Number of reflections | 31953 | 3148 |
<I/σ(I)> | 34.1 | 2.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 15.5 | 15.2 |
CC(1/2) | 1.000 | 0.930 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 293 | 0.1 M CAPS pH 10.5 and 29% PEG 400 |