6AOQ

Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	H 3 2
Unit cell lengths	100.614, 100.614, 382.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.350
R-factor	0.21791
Rwork	0.215
R-free	0.26157
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o5n
RMSD bond length	0.010
RMSD bond angle	1.462
Data scaling software	HKL-2000 (712)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.350	2.350
Rpim	0.030	0.220
Number of reflections	31953	3148
<I/σ(I)>	34.1	2.8
Completeness [%]	99.9	100
Redundancy	15.5	15.2
CC(1/2)	1.000	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	293	0.1 M CAPS pH 10.5 and 29% PEG 400