6AO4
Crystal structure of the human gasdermin D C-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9800 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 47.750, 108.540, 45.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.050 - 2.901 |
R-factor | 0.236 |
Rwork | 0.232 |
R-free | 0.28970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ao3 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.195 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.300 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 5664 | 402 |
<I/σ(I)> | 17.5 | 1.8 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.67 | 7.19 |
CC(1/2) | 0.998 | 0.865 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 2.1 M DL-malic acid, pH 7.0 |