6ANW

Crystal structure of anti-CRISPR protein AcrF10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	73.774, 106.618, 95.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.675 - 2.486
R-factor	0.2202
Rwork	0.218
R-free	0.26910
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.642
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.113	0.096	0.458
Rmeas	0.128	0.110	0.523
Rpim	0.060	0.052	0.246
Total number of observations	57879
Number of reflections	13323	1407	1299
<I/σ(I)>	7.6
Completeness [%]	98.9	97.9	99.2
Redundancy	4.3	4.2	4.2
CC(1/2)		0.990	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	293	0.1 M HEPES (pH 7.5) and 1.4 M tri-sodium citrate